TRAPPC9基因对奶牛金葡菌乳房炎抗性性状的遗传效应

doi:10.11843/j.issn.0366-6964.2016.02.009

Abstract

Abstract:

The present study was designed to investigate the genetic effect of TRAPPC9 gene on resistance to Staphylococcus aureus (S.aureus) in Chinese Holstein cattle.A total of 517 Chinese Holstein cows with intact data of DHI，age and parity were collected from 6 farms of North China.Multiplex SnaPshot was used to analyze the genotype of 4 SNPs of TRAPPC9 gene，the specific PCR was used to detect S.aureus infecting cattle.Meanwhile，the contents of IFN-γ，IL-17，NF-κB and TNF-α in serum for each cattle were measured.Based on this，the genetic effects of the 4 SNPs in TRAPPC9 on mastitis resistance to S.aureus were analyzed by the least squares method of GLM model.The results indicated that IL-17，TNF-α and IFN-γ could be used as effective indicators for mastitis.With regard to the S.aureus negative Chinese Holstein cows，SNP4 (C2477531T) of TRAPPC9 had highly significant effect on SCS (P<0.01)，while the SNP showed a highly significant effect on IL-17 in S.aureus positive cows (P<0.01).These data imply that bovine TRAPPC9 gene could be a powerful molecular marker of mastitis resistance to S.aureus in Chinese Holsteins.

CLC Number:

S823
S813.3

FENG Wen，DONG Yi-chun，WANG Xiao，LIU Chao，WANG Xin，WANG Ya-chun，YU Ying. The Genetic Effect of TRAPPC9 on Mastitis Resistance to S.aureus in Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 276-283.

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The Genetic Effect of TRAPPC9 on Mastitis Resistance to S.aureus in Dairy Cows

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